Start-ups reaped the gains as investors shrugged off scare-mongering by Remain campaigners in the lead-up to the referendum in June, according to technology investment bank GP Bullhound.
More than 4,000 deals were made in 2016, a huge leap from the 2,858 in 2015 and 1,714 the year before, showed Capital IQ data analysed by the bank.
Before the Brexit vote 2,426 agreements were made, and another 1,583 were made after the outcome - showing that deals continued to flow despite the Leave outcome.
Hugh Campbell, GP Bullhound’s co-founder, told the Sunday Telegraph that wider global uncertainty, including Brexit and the election of Donald Trump had dampened the number of deals in the second half of the year.
Fiercer negotiations over company values between investors and entrepreneurs has also slowed agreements, according to Mr Campbell.
He said: “There’s clearly a marked increase in the quality of companies and the appetite to invest in them, it’s about agreement on price; it’s much harder to close a deal."
Later-stage companies seeking funds from institutions continued to be successful in the second half of the year, added Mr Campbell.
However, private fundraising to smaller start-ups slowed.
High-profile technology deals last year included the £24bn takeover of ARM Holdings, £1.4bn sale of Skyscanner and a multi-million pound funding to food delivery firm Deliveroo.
Investment platform Seedrs also reported 2016 as its biggest ever year, with more than £85 million invested into 159 start-up deals funded.