Willie Rennie claimed voters who had previously backed Nicola Sturgeon are switching to Lib Dems
Launching his party's manifesto Mr Rennie pledged to halt a second independence referendum "full stop", insisting voters are "spitting mad" about Ms Sturgeon's drive towards another ballot.
The MSP claimed Scots across the country faced a "straight choice between the Liberal Democrats and the SNP" on June 8 and predicited tactical voting could give his candidates the edge in marginal constituencies such as East Dunbartonshire, Edinburgh West and Caithness.
Edinburgh West has become a particularly bitter battle as Lib Dem Christine Jardine hopes to seize the seat in the wake of the controversy surrounding Michelle Thomson, who was suspended by the SNP amid a police probe into property deals.
Last week a row erupted after SNP activist Simon Hayter falsely claimed Ms Jardine had campaigned during the Manchester terror attack general election truce.
She had in fact been at the funeral of her husband Calum Macdonald in Clydebank.
Mr Rennie pledged to halt a second independence referendum in his manifesto
We have got a positive campaign across the country where we are saying people can stop the debate on independence.
Willie Rennie, Scottish Lib Dem leader
Material for Nationalist candidate Toni Giugliano also criticises Liberal Democrat MSP Alex Cole-Hamilton over a probe into election spending and Ms Jardine for previously standing in other seats.
It also features a bar chart claiming only the SNP can beat the Tories in the seat, but this ignores Mr Cole-Hamilton's first-past-the-post victory last year and is based on a regional list seat, which uses a form of proportional representation.
Speaking in Edinburgh Mr Rennie said: "I afraid the SNP campaign in Edinburgh West has gone very low, but you know we have got a positive campaign across the country were we are saying people can stop the debate on independence, they have the right to reject a bad deal on Brexit, mental health and education those are positive issues that we are doing very well on.
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"Some of my friends in the party have to go through quite a lot of difficulties in their election campaigns so to see them overcome personal, vicious campaigns is a joy, but it's more to see them overcoming it than revenge on individuals who are pursuing a negative and aggressive campaign."
Manifesto pledges include a Brexit deal referendum, add a penny to income tax on dividends to invest £35 million in mental health, end the 1 per cent public sector pay cap, and scrap the two-child cap on tax credits and associated "rape clause".
The party has also promised to retain the triple lock for pensions, guarantee the rights of EU citizens and reintroduce post-study work visas.
Mr Rennie yesterday [WEDS] said his party will not back another referendum on independence no matter the circumstances of Brexit.
Mr Rennie claimed voters were 'spitting mad' about Nicola's drive towards a second referendum
UK leader Tim Farron has already said Lid Dems will not make any coalition deals and Mr Rennie ruled out any potential “confidence and supply deal” with Labour or the Tories.
He added: "I've been astonished at the rage that there is out there, including from some people who did vote for the SNP last time, and they want to stop it now.
"They are just spitting mad at the SNP at how they have banged on about independence all the time and think it's got to stop, and they are going to use this election to do so."
Responding to the manifesto launch, Tories highligted social media posts by Dundee West Lib Dem candidate Jenny Blain, who said in 2014 that she would be voting Yes.
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Ms Blain, a "pagan with an interest in shamanic rituals" wrote a blog claiming Scotland had been “betrayed” by the 1707 Act of Union and poetry backing the break up of Britain.
Tory candidate Kirstene Hair said: "If Willie Rennie wants to re-brand himself as the defender of the Union, he should explain why he's backing independence-supporting candidates."
An SNP spokesman said: "We're running a positive campaign to stand up for Scotland against an increasingly right-wing Tory government."