The Birmingham Edgbaston MP, who served as the chairwoman of Vote Leave, said £100m would go towards health services following a grilling on Sky News’ All Out Politics.
She was forced to defend the campaign’s battle bus which had the words “we send the EU £350million-a-week – let’s fund our NHS instead” emblazoned across it.
Ms Stuart said: “But that’s the amount we lost control of… we said £100m of that should go straight into the NHS specifically. £350m is what we lost control over, to make the decision on how to spend it.
“A huge chunk of it I hope will go into the NHS.”
Gisela Stuart defended the Leave campaign's battle bus as Theresa May triggered Article 50
A huge chunk of it I hope will go into the NHS
Speaking about a rumoured Brexit bill of up to £60billion, she said the Government would have to look at their “liabilities” and be “grown up” about paying anything.
She said: “You have to negotiate what is the proper price, what people voted for at the referendum was to bring an end an automaticity of the contribution.”
Speaking ahead of the triggering of Article 50, she called for a mutual “respect” from both sides during negotiations.
Ms Stuart said: “I think the key thing is both sides will start to look at themselves in a different way. The United Kingdom can start a process of national renewal as we are responsible for all policy areas.
Brexit day LIVE: images from around the country as Britain invokes Article 50 Wed, March 29, 2017
The country reacts as Theresa May officially invokes Article 50, and begins the process of Britain leaving the European Union
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Prime Minister Theresa May announces in the House of Commons that she has triggered Article 50, starting a two-year countdown to the UK leaving the EU
The Brexit bus came under fire during the campaign
“But the European Union itself has to start a process of deeper political integration to make their single currency work.
“And that process on both sides are realistic – respect each other and actually realise that we can both emerge stronger from this. Then, I think we can get a good outcome.”
As Article 50 was triggered, Mrs May made a rallying call to unite all sides of the Brexit debate in the UK.
She said: "Now is the time for us to come together, to be united across this House and across this country to ensure that we work for the best possible deal for the UK and the best possible future for us all."
Mrs May told the House of Commons: "This is an historic moment from which there can be no turning back."
“This great national moment needs a great national effort. An effort to shape a stronger Britain.
“Let us come together and work together. Let us choose to believe in Britain with optimisms and hope. If we do we can together make a success of this moment.”