Donald TRump has pledged to deport millions of illegal US immigrants during his presidency
The raids were conducted in at least 12 states and were "routine and consistent with regular operations carried out by US Immigration and Customs Enforcement" he said.
The operations targeted Midwest, Los Angeles, New York, North Carolina, South Carolina, Georgia and San Antonio, Texas.
Of the 41 people arrested in New York City and surrounding areas, 93 per cent had criminal convictions, although only 45 per cent of the 51 people arrested in the San Antonio had criminal records.
Among the 190 people arrested in Georgia, North Carolina and South Carolina, there were 17 people who had no criminal convictions or a prior order to leave the country, Reuters reported.
Rights activists have refuted Mr Kelly's claim that the arrests were "business as usual", saying the operations were far more sweeping than anything conducted under Barack Obama's administration.
Shiu-Ming Cheer, senior staff attorney at the National Immigration Law Center, said: "Now it seems like anyone could be arrested.
"The level of fear and anxiety is much higher than I've ever seen it."
Mr Kelly said in a statement that the operation targeted people who had criminal records or had flouted immigration laws.
Three quarters of those arrested had criminal records, he said, ranging from convictions for homicide to drink driving.
According to Immigration andCustoms Enforcement (ICE) some had ignored final orders of deportation.
Guatemalan immigrants deported from the United States arrive on a ICE deportation flight on February
Former President Obama was criticised for being the "deporter in chief" after he kicked out over 400,000 people in 2012, more than any president in a single year.
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In 2014, Obama's homeland security chief issued a memo directing agents to focus on deporting a narrow slice of immigrants, namely those who had recently entered the country or committed serious felonies.
Immigrants who were arrested for driving under the influence of alcohol, for example, were treated as lower priorities for deportation.
Republican President Donald Trump promised to deport two to three million migrants with criminal records on taking office.
At a news conference with Canadian Prime Minister Justin Trudeau on Monday, Trump said his administration had "really done a great job" in its recent arrests of immigrants.
Mr Trump said: “We're actually taking people that are criminals, very, very, hardened criminals in some cases with a tremendous track record of abuse and problems.”
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