The famed 44-year-old, whose real name is Marshall Mathers III, unleashed the vitriolic attack in a profanity-laced verse on the song No Favours, which features on Detroit rapper Big Sean’s upcoming album I Decided, which is out next week.
The song contains the lines: "I'm anti, can't no government handle a commando/ Your man don't want it, Trump's a b**** I'll make his whole brand go under.”
The track also touches on other social issues such as alleged police brutality, racism in the US and the Flint water crisis.
Eminem performs during 2016 Lollapalooza Brazil Day
A number of artists and activists within the US hip-hop community are said to be gearing up for a political battle with the newly installed Republican administration, with particular concerns being voiced about the appoint of Senator Jeff Sessions, who some see as being detrimental for reform of the criminal justice system, should be confirmed as the new Attorney General.
It is not the first time the Slim Shady singer has slammed Mr Trump.
US President Donald Trump has come under fire from rapper Eminem
I'm anti, can't no government handle a commando/ Your man don't want it, Trump's a b**** I'll make his whole brand go under
Eminem raps on the Big Sean track No Favours
Eminem recorded the eight-minute freestyle track Campaign Speech in October last year where he comments on the presidential election and attacked supporters of Mr Trump.
"But you should be afraid of this dang candidate/ You say Trump don't kiss a** like a puppet/ 'Cause he runs his campaign with his own cash for the fundin' and that's what you wanted / A f***** loose cannon who's blunt with his hand on the button/ Who doesn't have to answer to no one."
High profile personalities within the US hip-hop community have had an ambiguous relationship with the 45th President.
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Eminem in action during 2016 Lollapalooza Brazil Day
Being President Trump: Key Moments
Fri, February 3, 2017
Inside Donald Trump's first week in the White House.
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Trump signs an order to review the Dodd-Frank Wall Street to roll back financial regulations of the Obama era.
Donald Trump appears to have lost his appeal with the US hip-hop community
The 70-year-old was initially respected by key figures in hip-hop, often being invoked to signify wealth and power but recently the tide began to turn.
Lil B sent a text to a US broadcaster before the election saying: "I will be voting for Hillary yes!!! If not bernie then her.
Eminem features on the upcoming Big Sean album
"Even tho I am republican cuz im a businessman but my views and heart are democratic and for the people."
Before the election, Charlamagne Tha God, who co-hosts one of the most popular hip-hop radio shows in America, said that with Trump as the GOP nominee, people "can't afford" not to vote for for Democrat Hillary Clinton.