Pilots working for the travel group Thomas Cook will stage a strike on Saturday as part of a dispute over pay.
Their trade union, the British Airline Pilots’ Association (Balpa), confirmed the strike after five days of talks failed to resolve the dispute.
A strike on 8 September was the first by pilots at the company since 1974.
Further walkouts, on 29 September as well as 6 October, are also in the pipeline.
The next strike will last for 24 hours.
The dispute is over a 1.5% pay offer which Balpa has said is unreasonable and amounts to a real-term pay cut.
The union says it wants a rise in line with inflation – currently running at 2.9% on a CPI basis – and better travel facilities.