Gulnur Aybet appeared on Sky News to shut down claims of manipulation and irregularities at the ballot box.
Her defence comes after Bulent Tezcan, deputy chairman of the People's Republican Party (CHP), the main secularist opposition, called for the referendum result to be annulled.
He said the party had received complaints from many regions that people had been unable to vote in privacy and said that some ballots were counted in secret.
But Ms Aybet maintained the position there had been no wrongdoing by the Turkish Government.
Gulnur Aybet blasted those attempting to taint President Erdogan's referendum win
I would really like them to give me an example of a recent European election where you had 85 per cent of the electorate turn out to vote
She told the news channel: “I think the observers really need to do some better explaining with examples about the report because, for example, they cited that some people had been unable to vote as one of the irregularities that they saw.
“I would really like them to give me an example of a recent European election where you had 85 per cent of the electorate turn out to vote and which is exactly what happened with this referendum.
“And when they say the campaign was imbalanced, I mean in every election and referendum, one side that campaigns is the government.
“Otherwise, are they saying that it’s only the opposition who should be campaigning?”
A look back on the shocking Turkish military coup that left 294 dead
Wed, July 27, 2016
Get Quotes on Home Insurance
The violent military coup to overthrow Turkey's President Erdogan has 'failed' leaving at least 104 dead and more than 1,500 wounded
1 of 42
A police officer talks with the soldiers involved in the coup attempt after they surrendered
Protesters demonstrating against the referendum result in Turkey
She also bashed critics for questioning Turkey’s legal frameworks for dealing with referenda.
“They also said in the report that the legal framework for referenda was inadequate – so are they actually saying that all eight referendums that were held in this country are null and void?” she asked.
“And why haven’t they made this point in previous referendums? So I really have issues with that report and I would really like to see them give specific examples.”
The comments come as after protests broke out across the country in the hours following the referendum result, which gives more powers to the Turkish leader.
Mr Tezcan told a press conference the High Electoral Board's (YSK) decision to accept unstamped ballots was also clearly against the law.
"At the moment it is impossible to determine how many such votes there are and how many were stamped later," he said.
"This is why the only decision that will end debate about the legitimacy (of the vote) and ease the people's legal concerns is the annulment of this election by the YSK.”