Struggling to read the question aloud, 56-year-old host Bradley had to attempt to read the quiz several times to 59-year-old contestant Fern Britton.
Trying to hold back the tears, The Chase star said: "Cock shot. Here we go. Alright. Cock shot and beaver," before pausing completely.
Fern offered encouraging words, saying: "You can do it," before Bradley replied: "I can, if that lot would shut up and give me half a chance."
"'Cock shot' and 'beaver' are terms in what game?," said Bradley, before erupting in laughter once again.
The Chase: Bradley Walsh LOSES it over 'cock shot and beaver'
The Chase: Bradley Walsh could not contain his laughter
'Cock shot' and 'beaver' are terms in what game?
The possible answers offered were Backgammon, Ker-Plunk and Twister, but the Ker-Plunk option had the host in stitches.
Fern answered correctly with an answer of Backgammon, but 51-year-old chaser Mark Labbett hit incorrect answer Ker-Plunk leaving Bradley in tears, asking: "You are kidding me right? That's brilliant. That was great!"
Fans of the show were in hysterics at Bradley's reaction, as the presenter offered one of his classic laughter break-downs.
One viewer tweeted: "Bradley Walsh cracking up makes #TheChase worth watching."
The Chase: Bradley Walsh in hysterics
The Chase: 'Shut up!' Fern Britton intervenes as Bradley Walsh COLLAPSES over beaver gaffe
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"How did @BradleyWalsh not laugh then because I'm in tears #TheChase," posted another.
A third said: "HAHAH that question on The Chase 'what game are the terms cock-shot and 'beaver from?"
"'Cock shot' and 'beaver' on The Chase, lovely stuff," laughed a fourth.
Two questions from home, players Matthew Hoggard and Fern won £42,000 to split between their respective charities – Genesis Research Trust and Rainbows Children's Hospice.