The 56-year-old host got down on his knees and did an impression of Hervé Villechaize's Tattoo.
Speaking to contestant Ian, a 44-year-old project manager from Bridgend, Bradley said: "You're a very deliberate player, not so good in the cash builder, but great in the final chase."
Introducing the possible chasers, the ITV presenter said: "Or is it the Sinhaman? You can see him on The Chase, on comedy shows, and at the start of re-runs of Fantasy Island shouting, 'The plane, boss, the plane'."
Ian looked uncomfortable, but his fellow players Coral, 70, Natasha, 34, and Richard, 22, were in hysterics.
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The Chase: Bradley Walsh MOCKS Paul Sinha and compares him to James Bond villain Tattoo
The Chase: Bradley Walsh mocked Paul Sinha
Or is it the Sinhaman? You can see him on The Chase, on comedy shows, and at the start of re-runs of Fantasy Island shouting, 'The plane, boss, the plane'.
"Boss the plane, the plane boss, the plane," Bradley continued, having dropped to the floor to impersonate the 3ft 11 actor.
Paul, 46, later appeared in his trademark white tuxedo, identical to the one worn by Tattoo.
Viewers seemed to love the impression, with one viewer posting: "Absolutely hilarious Bradley Walsh."
"Haha Bradley Walsh is class #TheChase," posted another, before a third asked: "What is Bradley doing on his knees? haha #TheChase."
"Did Bradley just do Bond on #TheChase??!" posted a fourth, as other fans posted pictures of the bond baddie.
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The Chase: Bradley Walsh as Hervé Villechaize's Tattoo
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Tonight's show also introduced the word "meme" when a contestant was asked about an image with text that "went viral" last year.
Bradley asked: "A popular internet meme features which footballer inserting himself into other people’s celebrations?"
Answering incorrectly, the hopeful picked Wayne Rooney, with the correct answer being John Terry.
One viewer tweeted: "Bradley Walsh just said meme on the chase I love 2017," before another joked: "There was a question about memes on The Chase THIS IS MY CALLING."
A third laughed: "Just woke up from a nap and saw that memes are on The Chase now."