Before taking to the stage tonight, the 30-year-old relaxed on the pitch at the NRG stadium in Houston, Texas where the New England Patriots will play Atlanta Falcons.
The Bad Romance singer seemed oblivious to the wardrobe malfunction as she lifted her left leg up and revealed more than she would have bargained for.
Her flesh coloured knickers were on show for all to see as she stretched out on the field wearing a pair of black high heels.
The camera flash also revealed a little more than expected as her nude bra was clear to see underneath her long-sleeved black top.
Lady Gaga flashed her knickers at the Super Bowl LI
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She matched the outfit with a short skirt that featured a long train which showcased her toned lean pins.
Lady Gaga – real name Stefani Germanotta – will be the eight solo artist to perform at the halftime time show tonight.
Ahead of her anticipated performance, she spoke of her excitement to be a part of the historic moment at a press conference last week.
She vowed to the crowds that she would put on a show they would “never forget”.
Lady Gaga flashed her underwear as she relaxed on the pitch in Texas
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The Born This Way hitmaker also said her favourite halftime show memory was watching Michael Jackson in California in 1993.
The musician said: "Watching Michael Jackson do the halftime show is one of the fondest memories that I have.
"He encapsulates everything that you love about a great performer. You watch him go on stage and you can see he's getting his head into the zone and he's having a true defining moment. It's what it must feel like to be a champion."
The last time the American football match was held at this stadium, Janet Jackson suffered her infamous nip slip on stage 13 years ago.