Nicola Sturgeon has defended spending almost £140,000 on her failed Brexit bid
Tory backbencher Maurice Golden said the Scottish Government's intervention last month Supreme Court battle was just the tip of the iceberg when it came to breaking up Britain.
Lord Advocate James Wolffe represented ministers and argued Holyrood was entitled to a vote before EU exit proceedings begin.
The Supreme Court rejected the argument on devolved administrations while also ruling against the UK Government, forcing MPs to be given a vote on the Article 50 process.
At the weekend it emerged £128,877 was spent on "external legal fees", while there was an £800 court fee to intervene in the case.
Mr Golden, a West Scotland MSP, asked the First Minister if she thought the £136,000 outlay was "worth while".
Lord Advocate James Wolffe represented ministers and argued Holyrood was entitled to a vote
He added: "This Scottish National Party Government will say and do literally anything that it thinks will further its goal of tearing our Union apart, and it does not care how much Scottish taxpayers’ money it squanders in the process.
The SNP Government puts its own interests first, not those of Scotland
Tory backbencher Maurice Golden
"The £136,000 that I mentioned is one example of the tens of millions of pounds that the SNP Government spends on policy decisions that it believes will promote separation.
"No one should be under any illusions about the fact that the SNP Government puts its own interests first, not those of Scotland."
It emerged £128,877 was spent on 'external legal fees' in the unsuccessful bid
But Ms Sturgeon hit back saying her administration would not have needed go to court action if the UK Government did not pursue a case they were not going to win.
She also mocked Mr Golden for raising the issue of cost when the Conservative ministers had refused to reveal how much they have spent on the case.
Brexit: Which parts of the UK had the majority vote? Fri, February 17, 2017
Much of the North East of the United Kingdom voted to leave the European Union including Sunderland, Gateshead, Darlington, Durham, Hartlepool, Middlesbrough, Stockton, Redcar and Cleveland, North Tyneside and South Tyneside, and Northumberland. Newcastle was the only borough to vote to remain.
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GREAT YARMOUTH: The town of Great Yarmouth on the East Coast of England voted by 72% to leave the European Union.
Ms Sturgeon has defended herself against claims that taxpayers' money was squandered
She added her government’s intervention was “necessary to force the UK Government to enact the legislation that is currently going through the Westminster parliament before the triggering of Article 50”.
“The case also raised fundamental issues about the rights of people in Scotland and the role of this parliament,” she added.“So yes I do think it was absolutely right that this government, like the government in Wales, defended our interests in what was the most important constitutional law case for many, many years.”