With less just four days till the election, the First Minister was quizzed by voters venting their fury at a decade of SNP rule at Holyrood as she joined Lib Dem leader Tim Farron.
The Nationalist leader faced calls to resign and saw her administration branded “hopeless” amid a backlash over plummeting schools standards and a beleaguered health service.
Challenging Ms Sturgeon one BBC audience member in Edinburgh said: “Do you accept that you miscalculated the mood of Scotland by calling for another independence referendum?”
But defending plans for another poll over quitting the EU she replied: “Sometimes in politics you have to do you what you think is right.
Nicola Sturgeon last night failed to accept she had blundered with her demands for another independe
“My position on this is reasonably straightforward when you strip it all away we face not just Brexit put perhaps a very extreme form of Brexit that could have implications and consequences for life in Scotland for generations to come.”
Responding to plummeting approval ratings she replied: “All the polls suggest the SNP is on track to win this election in Scotland fairly convingcingly.”
Mr Farron too faced a fierce grilling over his calls for a second Brexit referendum and opposition to more internet monitoring to clamp down on jihadis.
The committed Christian also repeated his view that he did not think homosexuality is a sin after again being faced with questions about his beliefs.
When asked about his views, he added: “I’m not running to be Pope. I am political leader not a religious one.”
Nicola Sturgeon's comical moments on the campaign trail Mon, May 22, 2017 Play slideshow PA 1 of 13
SNP leader Nicola Sturgeon stops for an ice cream after making an election campaign visit to Scottish Gas HQ in Granton, Edinburgh
The leaders took it in turn to take questions from the crowd.
Get Quotes on Home Insurance
The programme was presented by the BBC’s former political editor Nick Robinson, who faced calls to be sack from Yes supporters over alleged bias during the 2014 referendum.
Question Time veteran David Dimbleby was preparing for Thursday night’s election coverage.
The programme was originally due to take place just before 6pm on Sunday less than 24 hours after the London terror attack.
Meanwhile, Labour’s campaign was plunged in fresh chaos last night [MON] after the Welsh First Minister said the party would scrap the Barnett Formula.
Carwyn Jones said the party’s Welsh manifesto pledges to ditch the system which calculates funding given to Scotland, Wales and Northern Ireland.
After the show rivals urged voters to send Ms Sturgeon a message on Thursday to take her "demands off the table".
Scottish Tory MSP Ross Thomson said: “Once again, we’ve seen the First Minister taken to task on her party’s dreadful record on education.
The Nationalist leader faced calls to resign
"She struggled once again to explain why, after 10 years in government, standards in our schools have slipped so badly.
“The reason is that this SNP Government has spent 10 years obsessing about independence.”
Labour General Election campaign manager James Kelly said:"This was a tough night for the First Minister.
"No wonder Nicola Sturgeon spends so much time up in her helicopter, because when she comes into contact with the ordinary people of Scotland it becomes clear that the majority don't want another divisive independence referendum.
"It's time for Nicola Sturgeon to listen to the majority of Scots, who want her to get on with the day job of improving standards in our schools and hospitals instead of constantly trying to break up our country.”