Leader Kezia Dugdale hit out a plummeting standards during the Nationalists' decade in power at Holyrood amid repeated demands for another referendum.
She stepped up the attack with a new election broadcast which warns Nicola Sturgeon offers "the same old answer for everything" – another vote on separation.
Ms Dugdale condemned the First Minister for presiding over cuts to teacher numbers and local government funding.
Kezia Dugdale has accused the SNP of 'playing a broken record' over independence
She said the Scottish Government has also failed to tackle A&E waiting times and had presided over a real-terms pay cut for nurses.
Nicola Sturgeon's answer is another referendum – she is playing a broken record
Ms Dugdale said: "On Thursday, people can send Nicola Sturgeon a message that she should focus on the day job, rather than forcing another independence referendum that people in Scotland don't want.
"After 10 years of the SNP campaigning for independence, Scotland's schools and hospitals have suffered.
"Nicola Sturgeon's answer is another referendum – she is playing a broken record."
Dugdale condemned the Sturgeon for presiding over cuts to teacher numbers and local funding Nicola Sturgeon's comical moments on the campaign trail Mon, May 22, 2017 Play slideshow PA 1 of 13
SNP leader Nicola Sturgeon stops for an ice cream after making an election campaign visit to Scottish Gas HQ in Granton, Edinburgh
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Despite polls suggesting her party will again be pushed into third place by the Tories, Ms Dugdale also insisted Labour is best-placed to beat the nationalists "in the majority of seats across Scotland".
Elswhere, today Scottish Lib Dem leader claimed thousands of Unionists voters were poised to tactically back his party in key marginal seats.
Mr Rennie said: "People are just fed up with the SNP.
"They want to put an end to the constant threat of another divisive independence referendum and see this election as a big opportunity to achieve that."
'People can send Nicola Sturgeon a message that she should focus on the day job' says Dugdale
Deidre Brock, SNP candidate for Edinburgh North and Leith said: “All Labour has to offer voters in Scotland is opposition to the SNP, and parroting Tory slogans – nothing positive.
“The truth is Labour cannot win this election in Scotland and any votes for them risks letting Tory MPs in by the back door."