The Pirates of the Caribbean actor accepted his gong for Favourite Movie Icon at the event that was held at the Microsoft Theater in Los Angeles.
During his speech, the crowds cheered and screamed as he thanked his fans who “stood by him” and “trusted him”.
The 53-year-old began: “I came here for one reason, and one reason only. I came here for you, the people, who through whatever good times or bad have stood by me and trusted me.
“Thank you. You very graciously invited me here once again tonight. I appreciate it. You have no idea how much I appreciate it.”
Johnny Depp thanked his fan who 'stood by' him during Amber Heard divorce
Johnny Depp said a tribute to his late mother
I came here for you, the people, who through whatever good times or bad have stood by me and trusted me
He sounded emotional as he continued speaking: “I was very deeply affected by the kindness of your recognition and your well wishes to me and my family.
“Why it is especially meaningful for me to be here in front of you is to say thank you and tell you that I truly feel the need to thank?
“Because in all honesty we all know that none of us, especially me, would not be standing up here if it wasn't for all of you.”
The A-Lister then looked above and shouted a tribute to his late mother: “Give ‘em hell Betty Sue.”
His appearance at the bash comes just days after his divorce from Amber was finalised, eight months after they announced their split.
Johnny Depp said he was 'affected' by his fans 'kindness' that they had shown him
The People's Choice Awards
Thu, January 19, 2017
Blake Lively, Gwen Stefani & Jennifer Lopez lead the best dressed list at the People's Choice Awards in LA.
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Blake Lively, Gwen Stefani & Jennifer Lopez at the People's Choice Awards
Johnny Depp and Amber Heard's divorce has now been finalised, eight months after they split
The Edward Scissorhands star has reportedly agreed to pay his ex-wife $7 million, which she has pledged to donate to two charities.
The 30-year-old Rum Diaries actress filed for divorce against Johnny in May last year and accused the actor of domestic abuse, which he denied.
The split came just three days after his mother passed away on May 20 in Los Angeles at the aged of 81.
Elsewhere at the award show, Dwayne ‘The Rock’ Johnson was caught on camera giving his co-star Kevin Hart the middle finger.
Blake Lively was seen dazzling on the red carpet in a thigh-skimming dress and Jennifer Lopez looked incredible in a figure-hugging dress that clung to her curves.