Delving into its unpopularity after it's move from the BBC to Channel 4, and loss of Mary Berry and much-loved hosts Mel Giedroyc, 48, and Sue Perkins, 47, it proved a touchy subject.
Berry later won the award for best judge, and people's hearts melted as she was presented with the title by two of the youngsters from Gogglesprogs.
"So, good evening. We are live on ITV and welcome to the 22nd National Television Awards," said 43-year-old Dermot.
"22 years and we still haven't been bought by Channel 4. Incredible. What are we doing wrong?"
NTAs 2017: Host Dermot O'Leary throws MAJOR shade at Great British Bake Off in opener
22 years and we still haven't been bought by Channel 4. Incredible. What are we doing wrong?
The camera then panned to the 81-year-old former Bake Off judge, but she looked far from impressed.
One viewer tweeted: "Mary Berry didn't look impressed by Dermot's Channel 4 gag, like Dermot on these though."
"'22 years and we still haven't been bought by channel 4' *camera on Mary berry* I'm in tears," laughed another.
On later winning her award, one viewer wrote: "Yeeeessssss Mary berry!!! Yeeeessssss!!! In your face channel 4!! #NTAS #GBBO."
NTAs 2017: Mary Berry won the award for Best TV Judge
Inside the National Television Awards 2017
Wed, January 25, 2017
All the winners from the 2017 NTA's
1 of 66
Mary Berry reacts to winning the Best TV Judge during the National Television Awards
"Mary Berry should've had a top underneath that dress that said 'f**k you Channel 4' #NTAs," posted another.
A third said: "Still can't believe Channel 4 were foolish enough to buy Bake Off without Mary Berry as part of the deal. That's just stupid. #NTAs2017."
"Oh that's absolutely lovely for Mary Berry that's she's won at the #NTAS And a standing ovation! She is a #NationalTreasure #GBBO," noted a fourth.