Tory leader Ruth Davidson hit out at the First Minister's "bulldozer" tactics in pushing for another poll on breaking up Britain.
Despite being told "now is not the time" Ms Sturgeon launched a marathon debate seeking Holyrood's consent to pursue a Section 30 order to hold a legally-binding poll.
The SNP leader wants a re-run of the divisive 2014 vote on separation to take place between autumn 2018 and spring 2019, while Theresa May has rejected the timing.
The Prime Minister has said Scots cannot make a judgment until they know how Brexit has bedded in.
Her minority administration is expected to win tomorrow's ballot of MSPs with the help of the pro-independence Greens despite opposition from the Conservatives, Labour and Lib Dems.
However Presiding Officer Ken Macintosh has already stated that motions passed by Holyrood are not binding.
During yesterday's three-hour debate Ms Sturgeon again signalled she was willing to negotiate over timing but argued attempts to block a referendum "runs the real risk of undermining the democratic process".
But Ms Davidson lambasted the First Minister for breaking her pledge the last poll would be a "once in a generation" event and that another one would not happen without a shift in public opinion.
First Minister Nicola Sturgeon was warned that Scots are 'sick to death' of her indyref demands Nicola Sturgeon's most outrageous quotes Mon, March 13, 2017
The nationalist chief always has plenty to say
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She and other leaders also took aim at Green co-convenor Patrick Harvie for breaching his Holyrood election manifesto which stated a plebiscite "should come about by the will of the people and not be driven by calculations of party political advantage".
Most people in Scotland are sick to death of the SNP's games
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Ms Davidson highlighted that the Edinburgh Agreement – a deal between the Scottish and UK Government's paving the way for the 2014 ballot – had been described by Nationalists as the "gold standard".
Attacking the SNP's "bulldozer" attitude she said: "This today, this is not the gold standard – it's a tinpot approach to the biggest decision we could ever be asked to make.
Tory leader Ruth Davidson hit out at Ms Sturgeon's 'bulldozer' tactics in pushing for another poll
"Most people in Scotland are sick to death of the SNP's games.
"They don't want another referendum any time soon, just three years after the last one.
"But let's cut to the chase: the SNP's plan was not actually about trying to hold a fair, legal and decisive referendum.
"It was about a well-rehearsed game to put forward unworkable proposals, wait for Westminster politicians to point that out, then rush to any nearby microphone – angry face attached – to trot out the same old tired complaints."
Ms Sturgeon argues last year's SNP election manifesto gives her an "unquestionable democratic mandate" to press for a vote because Brexit amounts to "a significant or material change" in circumstances since 2014.
The First Minister wants a new referendum to take place between autumn 2018 and spring 2019
But critics insist that this is not the case because she failed to win a majority and did not provide an explicit commitment to hold another referendum.
Opponents also point out that Ms Sturgeon has ignored the will of the Scottish Parliament five times on issues such as a ban on fracking and scrapping SNP's controversial anti-sectarian football laws since last year's Holyrood election.
Ms Davidson said: "Why do they exclaim that the Westminster government should recognise votes in the Scottish Parliament when the Scottish Government does not do so?"
Scottish Labour leader Kezia Dugdale said Ms Sturgeon was focused on "the only thing that has ever really mattered to the SNP" – independence.
Ms Dugdale said: "Nicola Sturgeon wakes up every single day thinking of ways to engineer another referendum.
"Because leaving the UK is the only thing that matters to her. It isn't improving education in Scotland. It isn't lifting children out of poverty.
"It's independence. That will always come first and it always has."
She added: "Brexit isn't the motivation for another referendum – it's just the latest excuse."
Willie Rennie, the Scottish Lib Dem leader, warned having another vote in independence in response to Brexit would bring "chaos on chaos" and "divide" families, communities and friends.
He said: "We do not respond with a break from Europe with a break from the UK."
Ms Sturgeon motion states: "That the parliament acknowledges the sovereign right of the Scottish people to determine the form of government best suited to their needs and therefore mandates the Scottish Government to take forward discussions with the UK Government on the details of an order under Section 30 of the Scotland Act 1998."
It goes on to say that Holyrood should be given the power over deciding the question and timing of the referendum.
A series of opinion polls show the majority of Scots do not want a referendum before Brexit in March 2019.
Scottish Labour leader Kezia Dugdale claimed independence is the only thing that matters to the SNP
But Ms Sturgeon today insisted it would be "wrong, unfair and utterly unsustainable" for Westminster to stand in the way.
With Mrs May set to trigger the Article 50 formal leave process on March 29 she told MSPs: "As a country, we can't avoid change.
"But we can choose what kind of change we want.
"The decision about the kind of country we are and the path we take can only be made by the people of Scotland."
Defending his position Mr Harvie said it was "absurd to suggest that we should not respond to and react to the fundamentally-changed circumstances we now find ourselves in."
He added: "The situation is changed not only by the EU referendum result, but by everything the UK government has done with it.
"The recklessness of holding that referendum to resolve their own internal squabbles, the utter lack of a plan and the disrespect shown to Scotland since then."