Part of the first series of the BBC hit was filmed at Levant Mine and annual visitor numbers have soared by 50 per cent to 100,000 since it was first broadcast in 2015.
Parking at the site in Cornwall was always free but now costs £1 an hour.
National Trust chiefs argued that by introducing parking fees they would be able to conserve and maintain the site.
The National Trust has been accused of cashing in on the Poldark craze
But locals say they do not want it to become a Poldark tourist attraction and that it should remain a sacred spot for the relatives of all those who died in the 1919 disaster.
Opponents say "public feeling is high" and the ticket machine has already been ripped out of the ground.
It has since been replaced but Ian Cooke, who has created a Facebook group against the move, said they hadn't given up the fight.
A ticket machine was installed at the mine
He said: "The National Trust have still not taken on board public feelings about the new charges as applicable to Levant, but hope they eventually see sense and abandon this policy as it applies to the very special case of Levant due largely to the disaster of 1919.
The National Trust, in their utter arrogance, have insulted us
"Public feeling against this new policy is running very high locally and, to prevent a repeat of unsightly and costly vandalism, I once again appeal to the National Trust to reconsider their parking policy and agree to grant an exemption for this location."
He added: "We don't want Levant Mine to be treated as a Poldark attraction."
Get Quotes on Home Insurance
Another local resident, who lost two ancestors in the tragedy, said: "The National Trust, in their utter arrogance, have insulted us.
The National Trust argues it needs the money for conservation purposes
"This is our land just as Land's End was before it was taken from us.
"Stop this wanton destruction and commercialisation of our Cornwall."
The National Trust said the new charges at Levant Mine were an important way of funding conservation and maintaining the site
Thu, September 10, 2015
Aidan Turner, star of Poldark in pictures
BBC/Mammoth Screen/Mike Hogan
1 of 11
Aidan Turner in Poldark
The parking tickets cost £1 an hour
Spokesman Ian Marsh said: "A terrible tragedy happened in 1919 where 31 men lost their lives there.
"We know people still come to pay their respects, those that descend from the miners that lost their lives, and we don't want to prevent that from happening in any way.
"Those people are able to park for free and while they're with us we want to hear their stories and hear their memories of the place."