While the majority of MPs in Parliament campaign for Remain, it is expected the House will vote overwhelmingly to activate Article 50 when it comes to a vote.
Discussing the issue, O'Brien said: "Do you see why the ultimate subversion of parliamentary democracy comes when you order an MP to vote for what he or she believes will harm his constituents.
"That's what we've done, guys. Great work.
"We've created a country now where people bleating ignorantly about democracy, sovereignty and parliamentary democracy have created a landscape in which we're going to ask our elected representatives to vote for something that they believe will hurt their constituents.
LBC • GETTY
The LBC host said the making MPs vote on Article 50 was a 'mockery of democracy'
"And if they don't, they'll be crucified."
O'Brien, one of LBC's most popular presenters, argued MPs were being forced into a corner by the ruling as he said they could not win.
He argued those who vote against triggering Article 50 would be "crucified" in certain sections of the media.
"And not literally crucified, but sticking pictures and phrases like 'Enemies of the people' for unelected judges, an independent judiciary, will be as nothing to what they do to MPs," he added.
We've created a country now where people bleating ignorantly about democracy
O'Brien's astonishing rant split viewers and listeners.
One posted: "I voted Remain, but after all this moaning and lies from mainly the Remain side, if it went to vote again I would vote LEAVE."
Another added: "Liberal scaremongering at its best. #Remoaners."
BREXIT: Supreme Court Ruling
Tue, January 24, 2017
Britain's most senior judges ruled that Prime Minister Theresa May does not have the power to trigger the formal process Article 50 for the UK's exit from the European Union without Parliament having a say.
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Issued by the Supreme Court of (top row, from the left) Lord Neuberger, Lord Mance, Lord Kerr, Lord Sumption, (bottom row, from the left) Lady Hale, Lord Clarke, Lord Wilson and Lord Hodge, who agreed with the majority decision that the Government could not trigger Article 50 without Parliamentary approval.
While a third social media user said: "It's people like James O'Bbrien that fuel divide in this country. The left are some of the most spoilt, angry intolerant people you can come across these days.
"It's their way or no way. People are starting to reject liberalism globally not just here, and people like James are the reason why."
Announcing the High Court's ruling was being upheld, Lord Neuberger, President of the Supreme Court, said: "The referendum is of great political significance, but the Act of Parliament which established it did not say what should happen as a result.
"So any change in the law to give effect to the referendum must be made in the only way permitted by the UK constitution, namely by an Act of Parliament.
"To proceed otherwise would be a breach of settled constitutional principles stretching back many centuries."