Manchester United face French side Saint-Etienne in the round of 32, with the fixtures taking place on February 16 and 22.
Morgan Schneiderlin and Memphis Depay have been omitted from the 27-man squad after leaving the club in January.
Man United stars bring out 4x4s to tackle farm track to and from training
Thu, February 2, 2017
Manchester United stars were forced to roll out their fleet of off-road vehicles as they made their way to and from training down a dirty farm track
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Manchester United stars are forced to take a farm track to and from training
Bastian Schweinsteiger is added to the squad after being left out for the group stages.
While goalkeeper Joel Pereira is also included despite suffering an injury this week.
Manchester United boss Jose Mourinho
According to the Manchester Evening News, Pereira has sustained a knee ligament damage and could be out for several months.
Dean Henderson is not in the squad despite being recalled from his loan at Grimsby as cover for Pereira.
Bastian Schweinsteiger has been included
A-List: De Gea, Romero, Valencia, Darmian, Bailly, Jones, Rojo, Smalling, Blind, Young, Carrick, Herrera, Fellaini, Schweinsteiger, Pogba, Mata, Lingard, Mkhitaryan, Martial, Rooney, Ibrahimovic.
B-List: Pereira, O'Hara, Fosu-Mensah, Tuanzebe, Shaw, Rashford.