Phil Jones has returned to Manchester United with injury
The defender will miss games against Germany and Lithuania this week.
The 25-year-old was hoping to make his first international appearance since November 2015 after an injury-disrupted period at Manchester United.
However, Jones has been forced to withdraw from the squad after picking up an injury ahead of Wednesday's friendly in Germany.
Phil Jones has pulled out of the England squad with injury
Phil Jones has been impressive for Manchester United this season under Jose Mourinho
A Football Association statement read: "Phil Jones has been released from the England squad after picking up an injury in training ahead of this evening's trip to Germany.
"The Manchester United defender has returned to his club and will not be part of the group that flies to Dortmund this evening.
"There are no immediate plans to call up a replacement but, with Gary Cahill suspended for Sunday's game against Lithuania, Gareth Southgate will assess his options following tomorrow's match."
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Jones, who has impressed under Jose Mourinho this season, had been looking to proving his worth with England this week.
"Looking forward to it," he said after United's 3-1 win at Middlesbrough.
"It has been a while because of injuries and not been playing. Hopefully I can have a good week there and do well.
"People write people off too quickly. When you are not playing you are out of sight and out of mind, that's football.
"But I have had a good run before a little knock and I am back now."