Brook last fought in September when he took a challenging fight up at middleweight against unbeaten Gennady Golovkin but won plenty of plaudits before he was stopped in the fifth round.
Spence meanwhile remains unbeaten in his professional career and has been tipped for greatness by many in the US.
The pair are expected to start their ring walks at 10.30pm local time but that will depend on the progress of a packed undercard.
George Groves tops the support action as he gets his fourth shot at a world title, taking on Fedor Chudinov for the vacant WBA super-middleweight belt.
David Allen fought for a global title but lost a split decision to Lenroy Thomas while Robbie Fowler's cousin Anthony marked his professional debut with a first-round knockout.
Two more of Eddie Hearn's Olympians were in action as Joe Cordina and Lawrence Okolie both scored similar round one stoppages.