The audience member on Question Time suggested that the SNP were attempting to blackmail the Government by demanding another referendum.
The former First Minister attempted to defend Nicola Sturgeon as the party were accused of restricting the work of government.
He said: “I’m just worried that the Scottish referendum operation has moved from being an issue about to the Union, to a sort of political blackmail.
“It’s become a noose on every UK government decision and I think if there is a second referendum and they lose it, there will be a third one, a fourth one, as soon as there is any major decision.
Alex Salmond was roasted by the audience member who mocked the SNP's independence plans
The audience member was applauded for his criticism of the SNP before the former First Minister attempted to protect his party’s decision.
He said: “In the SNP manifesto last year, Nicola Sturgeon on page 23 put in the commitment, that if there were a material change in circumstances, such as Scotland being taken out of the European Union against the will of the Scottish people, then the Scottish parliament should have a right to another referendum.
“That was a manifesto commitment. In the last few weeks around the budget and small businesses, what happens when the government try to ditch their manifesto commitment.
“That was in our manifesto, we were re-elected resoundingly with 47 per cent of the vote.
“The Scottish Parliament has every right to implement that manifesto.”
The Question Time panel discussed Nicola Sturgeon's demands for another referendum
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The Scottish referendum operation has moved to a sort of political blackmail
The row comes as the Scottish Parliament prepares to vote on Nicola Sturgeon’s demands.
The Greens are expected to support the SNP in Holyrood allowing Ms Sturgeon to approach the UK government for permission to hold another referendum.
The UK government however has insisted that it will reject demands after Theresa May reiterated that “now is not the time” to discuss another referendum in light of Brexit.
Mr Salmond said: “In answer to the gentleman, before Christmas Nicola Sturgeon put forward the position paper, ‘Scotland’s place in Europe’.
Live pictures: May meets Sturgeon during visit to Scotland Mon, March 27, 2017
British Prime Minister Theresa May met Scottish First Minister Nicola Sturgeon in Glasgow, following the announcement that Scotland is to hold a second Independence Referendum
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British Prime Minister Theresa May meets Scottish First Minister Nicola Sturgeon in Glasgow, following the announcement that Scotland is to hold a second Independence Referendum
“We said look, the way to resolve these differences, and there is no real attempt to do it just now. I mean Ireland is in stalemate, the Welsh are alienated, Scotland’s going to have another referendum, and England is still split 50/50.
“Her unifying the country isn’t going very well. When Nicola Sturgeon put forward the view that the way to do it was for the UK to stay within the single market place, to leave the European Union and to stay within the single market place and if that weren’t possible, then Scotland to have a special deal within the single market place, even if the UK were to leave it.
“That was the position papers. The Government has had it for nearly three months, Nicola Sturgeon hasn’t even had an answer.
“The attitude towards Scotland and the other devolved administrations has been contemptuous from the Westminster Government.”