Peers voted in favour of an amendment which would give Parliament a meaningful vote on the final Brexit deal.
The Government is expected to fight off the amendment during discussions next week, however Farage called for the chamber to be abolished following the result.
Peers backed the amendment with 366 voting in favour and with 268 voting against, which left the LBC host seething.
Farage snapped back at a listener who text in arguing that the House of Lords had a right to make to amendment to the Bill as it was part of British parliamentary sovereignty.
Nigel Farage hammered the House of Lords for their second amendment to the Article 50 Bill
He fumed: “I couldn’t care less about parliamentary sovereignty, I care about the sovereignty of the people, that is why we had a referendum on this.
“Rather than trusting our political class, in the House of Commons and the House of Lords who would never, ever, ever have voted to take us out of the European Union.
“We did it and we were promised that our decision would be final.”
The Prime Minister has previously insisted that MPs will have a vote on the Brexit deal but it would likely be a 'take-it-or-leave-it' basis.
Nigel Farage was left fuming as he called for the House of Lords to be abolished
I care about the sovereignty of the people
Get Quotes on Home Insurance
Farage targeted the House of Lords chamber as he accused them of purposely delaying the will of the people.
He added: “We are now going through months of wrangling to try and dilute, delay or even overturn the result of that referendum, that to me is plain blooming wrong!”
Last week the House of Lords voted to amend the Article 50 Bill in an attempt to protect three million EU nationals living in Britain.
Farage finished by calling for the House of Lords to be abolished for the way they have delayed the Brexit proceedings.
All the pictures from Brexit Bill Tuesday Tue, March 7, 2017
Theresa May is facing a second defeat on her Brexit bill Tuesday as the House of Lords votes on another change which would give parliament the final say on leaving the EU
AFP/Getty Images 1 of 20
Archbishop of Canterbury, Justin Welby, speaks in the House of Lords Chamber at the start of the third day of The European Union (Notification of Withdrawal) Bill
He said: “It’s stuffed of party donors, placeman, people who have frankly been promoted simply for party loyalty.
“It does not have the expertise, it does not have the geographical spread that it used to have.
“I will bet you that after this the calls to abolish the House of Lords will become very loud and clear.”