Mair asked Patrick Grady why he thought Scotland would get a seat at the table in talks with the European Union when it had not in the past.
Speaking on PM on BBC Radio 4, Mair said: “On being at the Brexit negotiating table, with all the MPs you had in the last parliament and the polls suggesting you may have fewer next time, you didn’t get a seat at the table and I’m wondering what makes you think you might get one next time."
Mr Grady hit back: “This is the choice that people in Scotland are facing, this is the manifesto that we’re launching today and this is what we’re asking for.
"We should hope that the United Kingdom government will recognise that.”
Eddie Mair asked Patrick Grady if a voice for Scotland in the Brexit talks was just a "hope"
Oh, it’s just a hope now?”
But Mair picked up Mr Grady on his answer and asked if it was a serious ambition. He said: “Oh, it’s just a hope now?”
The MP for Glasgow North tried to fight his corner as he said Scotland had the “right to demand” that the UK government listened to its request.
“It told us in 2014 that Scotland shouldn’t leave the UK, it should lead the UK,” he said.
“So now that we’re putting forward a position that respects the position of both of those referendums in 2014 and 2016, then the UK Government, we have the right to demand that the UK government listens to that.”
Nicola Sturgeon's comical moments on the campaign trail Mon, May 22, 2017 PA 1 of 13
SNP leader Nicola Sturgeon stops for an ice cream after making an election campaign visit to Scottish Gas HQ in Granton, Edinburgh
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Meanwhile, Nicola Sturgeon used her SNP manifesto launch on Tuesday to claim Brexit cannot be implemented in Scotland, stating "too much is at stake" if the country leaves the bloc.
She said the country needed to remain in the European Union for the sake of its young people, with leaving the single market a red line for the SNP.
And she reiterated her demands for a referendum on Scottish independence once the final terms of the Brexit deal is negotiated.