The 35-year-old This Morning presenter, who confessed that she was squeamish before the item, looked horrified as she watched a doctor inject a woman with botox live on the show.
As she introduced the beauty item, co-host Phillip Schofield warned viewers that there would be “needles and possibly a little bit of blood”.
“We’re about to see a couple of procedures demonstrated live. It involves needles and there may possibly be a little bit of blood. I’m hoping not a lot.” Holly looked nervous as she said: “I might hit the deck.”
She appeared uncomfortable throughout the segment and appeared to keep a distance from both Dr Tapan Patel and his patient Inga.
Holly was heard exclaiming shortly before Dr Patel inserted a needle into Inga’s face: “You’re going to do that right now?”
After Inga had been injected with a filler, Holly asked her: “Did that hurt? That must have hurt!” However, it looked like the patient had not felt it.
Holly Willoughby jokes about fainting on This Morning over a botox segment
Holly Willoughby jokes about fainting on This Morning
I might hit the deck
Meanwhile, Phil was the one left asking questions about the cosmetics procedures involving fillers and botox, the latter of which is the most common cosmetic treatment in the world.
Dr Patel said that although beauticians were doing the procedures, those considering treatment should ideally get them done by medical practitioners.
He said: “It’s a treatment that should be done by healthcare professionals,” and explained that it was “frowned upon” by the medical council to not go through someone qualified.
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Holly Willoughby looks uncomfortable talking about botox
The most cringeworthy moments from This Morning
Thu, September 29, 2016
The most controversial and cringeworthy moments from ITV's This Morning.
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This Morning features live botox
The doctor also advised that people should avoid discounts, although saying that prices varied across the country from £250 to £500 for the procedures.
Dr Patel said that people should also go to the same person for their treatments otherwise they could end up looking strange due to the various methods and products used by different people.
Phil told viewers of the ITV show: “It’s a face not a closing down sale.”
This Morning airs weekdays on ITV at 10.30am.