The suicide bomber, alleged to have been an Isis fighter, detonated the explosives just metres from a group of Syrian rebel soldiers.
The graphic footage appeared online on Tuesday and was filmed as the battle continued in the war-ravaged city of Raqqa.
In the 37-second video, gun fire can be heard as troops from the Syrian Democratic Forces (SDF) gather next to a tank.
The Isis fighter only blew himself up in the attack in Raqqa
Once Raqqa is liberated after Mosul, we will see the beginning of the end of this terrible caliphate
Sir Michael Fallon
The soldiers then begin to move into a more offensive position moments before the huge blast unfolds.
Then, a man can be seen charging around the corner from a countryside building where the men are stood, before he detonates himself.
Despite the horrific video, the fanatic was the only person killed in the barbaric attack, according to news agency Qasioun.
Syrian rebel soliders seemed unaware of the impending attack
The bomber is filmed running around the corner towards the men
The strike happened as soldiers continued the on-going operation “Wrath of Euphrates”.
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The SDF launched the military operation in November 2016 against the ISIS forces in Raqqa.
Troops have been surrounding Islamic State’s de-facto capital city and blocking off its key roads.
A huge blast can be seen, but only the Isis fighter was killed
If successful, critical routes used to supply the caliphates with resources would be completely cut off.
UK Defence Secretary Sir Michael Fallon believes the assault will be “completed by spring”.
Mr Fallon said: “I hope that isolation will be completed by the spring and then operations to liberate Raqqa itself can begin thereafter."
The fight against ISIS Fri, November 18, 2016
The battle against ISIS militants (also abbreviated as Daesh, ISIL, IS and Islamic State) continues in the Middle East.
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Forces battle against ISIS
He continued: "Raqqa is a much smaller city than Mosul but will clearly be defended very vigorously by Daesh and that means the operation to liberate Raqqa has to be very carefully prepared, as the operation for Mosul was.
"Once Raqqa is liberated after Mosul, we will see the beginning of the end of this terrible caliphate.”
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