Steve Wesson, 44, visited Strelley Hall, in Strelley, Nottinghamshire, during an investigation with UK Ghost Hunts in the hope of snapping a spook.
However the paranormal event company co-founder got more than he bargained for when he unwittingly filmed what he thinks could be the Virgin Queen silently hovering in a cellar archway.
Father-of-one Mr Wesson has since returned to where he believes the Tudor monarch appeared and claims to have possibly caught her on camera a SECOND time.
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Mr Wesson, from Kimberley, Nottinghamshire, said: "There is nothing plausible that it could be apart from an apparition.
"There was no one around, I just can't explain it.
"I have been through so many hours of footage and never seen anything like that before – it's just amazing.
An NHS worker claims he caught a ghostly figure at Strelley Hall in Nottinghamshire
Apparently Queen Elizabeth I slept above where this was taken as that's where the old rooms were
"Apparently Queen Elizabeth I slept above where this was taken as that's where the old rooms were.
"No-one ever talked about it as for some reason she wasn't supposed to be there – it was all a bit hush hush, some sort of secret.
"I've been through so many hours of footage and have never seen anything like that before, it's just amazing."
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The video shows a translucent figure appear for ten seconds before vanishing into thin air.
Fascinated by the footage Mr Wesson returned to the spot last week and claims to have caught Her Majesty on camera again.
He said: "I stood in the archway where the figure was after taking the picture and there was nothing there. I don't know if it's Queen Elizabeth I but it would be really cool if it was."
Steve Wesson 'freaked out after capturing Queen Elizabeth I's spirit on camera'
UK Ghost Hunts lead investigator Vicky Grant said: "I think the figure looks like a female as when you look closely it's almost like a flowing dress."
Mr Wesson said: "I remember it clearly popping up, it just appeared for a few seconds and then disappeared.
"It's just amazing – for something to just appear and disappear for no reason is quite shocking.
Queen Elizabeth I secretly stayed at Strelley Hall 600 years ago
"There are times when you still get freaked out and squeal like a girl but I usually have a bit of an explanation however this one still makes me shiver.
"I was excited as well as being freaked out – I was over the moon.
"You hear the stories about these sort of things but it's so hard to get them on camera.
"You can run cameras for so long and get nothing but to come across something that can't be explained is just incredible."
The Virgin Queen was silently hovering in a cellar archway
The team visited the hall on October 31, 2015, and returned the day after in a bid to find an explanation for the figure on screen.
Mr Wesson said: "We went back to the exact spot under the archway where the figure was seen to manifest and we tried to debunk it by trying different light settings to create shadows.
"We even tried to experiment with a real person but the figure was too whole and you could clearly see it was a person.
"We were overjoyed at our capture, something like this is extremely rare in the paranormal community and we feel very privileged that we captured this amazing piece of evidence. This will forever be one of our greatest captures."
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