Ben Gibson has replaced Chris Smalling in Gareth Southgate's England squad
England face Lithuania on Sunday in a World Cup qualifier.
Smalling has returned to Manchester United with an injury, following Phil Jones' return last week.
That leaves Gareth Southgate with just John Stones, Michael Keane and now Gibson with Gary Cahill suspended.
England star Lallana: The new 3-4-3 formation could be way forward
Security to be stepped up for England's clash with Lithuania after
Gareth Southgate has called Ben Gibson up to his squad for Lithuania
Southgate trialled a back three in the friendly against Germany and may have to use Eric Dier in defence if he plans to use that system again.
England top Group F after four games having beaten Slovakia, Malta and Scotland but drawn with Slovenia.
Lithuania are fourth with five points from their four games – a point more and a place above Scotland – in the six-team group.
England captain: Who is favourite to be named skipper?
Wed, March 22, 2017
Who is favourite to be named the next England captain?
The FA via Getty Images
Get Quotes on Home Insurance
1 of 7
Dele Alli – 12/1
Updated England squad:
Goalkeepers: Fraser Forster (Southampton), Joe Hart (Torino, on loan from Manchester City), Tom Heaton (Burnley)
Defenders: Ryan Bertrand (Southampton), Nathaniel Clyne (Liverpool), Ben Gibson (Middlesbrough), Michael Keane (Burnley), Luke Shaw (Manchester United), John Stones (Manchester City), Kyle Walker (Tottenham Hotspur)
Midfielders: Dele Alli (Tottenham Hotspur), Ross Barkley (Everton), Eric Dier (Tottenham Hotspur), Adam Lallana (Liverpool), Jesse Lingard (Manchester United), Jake Livermore (West Bromwich Albion), Alex Oxlade-Chamberlain (Arsenal), Nathan Redmond (Southampton), Raheem Sterling (Manchester City), James Ward-Prowse (Southampton)
Strikers: Jermain Defoe (Sunderland), Marcus Rashford (Manchester United), Jamie Vardy (Leicester City)