Eddie Jones will have plenty of injuries to assess ahead of the Six Nations opener
Prop Nathan Catt and back Alex Lozowski could make their international debuts alongside Mike Williams, who comes in as cover for an injury-ravaged back row.
Eddie Jones has also included James Haskell is also back in the squad despite a horrific concussion suffered earlier this month.
The Wasps flanker had missed the whole domestic season after having surgery on a toe injury but his return on January 9 lasted just 35 seconds when collided with Leicester’s Freddie Burns and was knocked unconscious.
England Rugby's 14-game winning streak
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Express Sport recaps England Rugby's unbeaten 2016
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England won every single match they played throughout 2016, and are currently on a 14-game winning run dating back to October 2015
However, Haskell – who was dominant during England’s tour of Australia – returns and is expected to start in the back row.
“It’s good to welcome back a number of players who weren’t with us during the autumn,” Jones said.
“Maro Itoje has done well for his club as has Jack Nowell.
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“James Haskell was magnificent for England last year and is a fierce player.
“Anthony Watson has been a fine player for England and is one the most prolific try scorers for the number of Tests he has played.
“We are looking forward to those guys coming in and challenging for a spot and adding strength.”
England will start their campaign against France at Twickenham in clash in which the defending champions could set the tone for the tournament.
And Jones wants his team to show none of the fear that haunted the team during the disastrous 2015 Rugby World Cup.
“At the start of last year’s Six Nations I probably didn’t realise the enormity of the tournament and how intense the rivalry is between the countries, so this year we’ll be better prepared for it and we are looking forward to being daring in our game against France on 4 February,” Jones added.
England Six Nations squad in full
Nathan Catt (Bath Rugby), Jack Clifford (Harlequins), Dan Cole (Leicester Tigers), Charlie Ewels (Bath Rugby), Ellis Genge (Leicester Tigers), Jamie George (Saracens), Teimana Harrison (Northampton Saints), Dylan Hartley (Northampton Saints), James Haskell (Wasps), Nathan Hughes (Wasps), Maro Itoje (Saracens), George Kruis (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Joe Marler (Harlequins), Matt Mullan (Wasps), Kyle Sinckler (Harlequins), Tommy Taylor (Wasps), Mike Williams (Leicester Tigers), Tom Wood (Northampton Saints)
Mike Brown (Harlequins), Danny Care (Harlequins), Elliot Daly (Wasps), Owen Farrell (Saracens), George Ford (Bath Rugby), Jonathan Joseph (Bath Rugby), Alex Lozowski (Saracens), Jonny May (Gloucester Rugby), Jack Nowell (Exeter Chiefs), Henry Slade (Exeter Chiefs), Ben Te’o (Worcester Warriors), Anthony Watson (Bath Rugby), Marland Yarde (Harlequins), Ben Youngs (Leicester Tigers)