The First Minister's personal approval ratings collapsed from +14 to -4 since September last year, a period which included her demands for another ballot on breaking up Britain.
An Ipsos MORI poll published today also found support for separation has slumped since a previous poll in March this year dealing a further blow to Ms Sturgeon's renewed constitutional battle.
It is the first time the SNP chief has recorded a negative rating by the pollster and makes her the least popular Holyrood leader.
NICOLA Sturgeon's popularity has plunged into the red for the first time
Ms Sturgeon was this week forced to deny at the SNP manifesto launch that she had become a “divisive” figure on the doorstep.
The survey suggests her party is on course to lose three seats to the Tories at the general election but still return a majority of Scotland’s MPs.
The SNP is on 43 per cent of those certain to vote on June 8 – down from 50 per cent at the 2015 Westminster election.
Ruth Davidson's Conservatives are running neck and neck with Scottish Labour at 25 per cent. The Lib Dem are on five per cent.
Ruth Davidson's Conservatives are running neck and neck with Scottish Labour
Translated into seats using Weber Shandwick’s Scotland Votes tool, this would see the SNP hold 51 of the 56 seats they took two years ago.
People are beginning to see through Nicola Sturgeon
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The Tories would increase to four. Labour and the Lib Dems would both double their current one each seat.
The Conservative vote share is up on its 2015 result of 14 per cent but down on other surveys which put it as high as 33 per cent.
But leader Ms Davidson received a higher satisfaction rating than Ms Sturgeon.
Scottish Tory deputy leader Jackson Carlaw said: "It’s clear, after a decade of failure in government and endless damaging agitation on independence, people are beginning to see through Nicola Sturgeon.
A poll says the backlash comes after over her crusade for independence
"This poll confirms that, in seats all over Scotland, it is the Scottish Conservatives who are best placed to bring the SNP down to size.
"Labour is now too weak, and with Jeremy Corbyn having confirmed he will open discussions with Nicola Sturgeon about a second referendum, they can’t be trusted to stand up against the SNP."
The survey for STV showed support for independence has fallen with 47 per cent in favour and 53 per cent against, versus a previous 50-50 split.
Half of respondents said they were dissatisfied with the way Nicola Sturgeon is doing her job as First Minister, while 46 per cent stated they were satisfied with her performance.
Sturgeon's top moments Fri, December 9, 2016
Scotland's First Minister Nicola Sturgeon in pictures.
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Nicola Sturgeon visits Glaxo Smith Kline.
There was also a slump across a range of key devolved areas. When asked who had the best policies on health and the NHS 34 per cent said the SNP compared to 48 per cent in 2015.
On education this fell from 49 per cent to 34 per cent and on crime stood at 33 per cent down from 33 per cent two years ago.
Theresa May was seen as the “most capable Prime Minister” by 42 per cent of respondents, while 40 per cent said Labour leader Jeremy Corbyn.
Mrs May’s popularity showed an even more dramatic slump than that of Ms Sturgeon.
Ms Davidson's net rating was +5, down 26 from a high of +12; Scottish Labour leader Kezia Dugdale was -2, up 8 compared to -10; and Scottish LibDem leader was +6 and Green Patrick Harvie +12.
Mrs May’s popularity showed an even more dramatic slump than that of Ms Sturgeon
Scottish Labour campaign manager James Kelly said it was "another encouraging poll" for his party.
He added: "A vote for Labour is a vote to reject a divisive second independence referendum and send Nicola Sturgeon a message to get on with the day job of fixing the crisis in our schools and hospitals."
SNP election campaign manager, Derek Mackay, said: "This poll shows that only a vote for the SNP can keep the Tories out.
"Labour can’t win the election in Scotland – and a vote for them just risks letting a Tory MP in the back door."
Ipsos MORI questioned 1,016 people between May 22 and 27.