Viewers took to Twitter to address the gaffe which centred around a hen party organised by Megan Macey (played by Gaynor Faye) and Leyla Harding (Roxy Shahidi).
The characters called upon Chas Dingle (Lucy Pargeter) and The Woolpack to host the event after they found out the original venue was infested with cockroaches.
The hens certainly made their presence known and were soon throwing up in the pub, before being kicked out for bad behaviour.
However, those at home were left questioning what time the event had been held, noticing that it still appeared to be daylight outside.
Emmerdale: The latest episode caused a stir last night
Emmerdale: It was later revealed that it was 4pm
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Who has a hen do with strippers at that time!
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As the long-running drama cut to another scene, a clock appeared in the background revealing it had only just turned 4pm.
"4 o’clock? Who has a hen do with strippers at that time or is the hospital visit the next day #Emmerdale @emmerdale?" one person questioned.
Two others continued: "How are they calling it a 'hen night' when its daylight outdoors," and: "The clock in Emmerdale says 4:20 and yet the hen party has been kicked out the pub."
Emmerdale: The hen party had caused a stir in the pub
Emmerdale: Another clock was later seen in the hospital
Another added: "So that was a lunchtime hen party then? Judging by how light it is in the rest of the village."
The instalment also focused on Chrissie White (Louise Marwood), who was hospitalised after a spat with her sister Rebecca.
Express.co.uk has contacted a representative for Emmerdale asking for comment.
Emmerdale continues tonight at 7pm on ITV.