With the Bridge Street market crowded with people protesting the council wanting to move the market site, Denise Fox (played by Diane Parish), new boy Keegan (Zack Morris) and Bex Fowler (Jasmine Armfield) were among characters trapped on a bus that ploughed into the crowd when the driver appeared to suffer a heart attack.
But a subtitle fail stole the limelight from the main EastEnders drama, as eagle-eyed viewers spotted an unlucky autocorrection.
When Bex and Louise were got into an argument with bullies at school and when Keegan, introduced earlier in the episode, called Bex a "fat little girl," fans noticed the subtitles had him calling her a "fat sk*t" instead.
EastEnders suffered a disastrous subtitle blunder on last night's bus crash episode
Subtitles accidentally called Bex a 'fat sk*t' instead of 'fat little girl'
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Susan Tully played Michelle Fowler until she left for America in 1995, she is now played by Olivier Award-winning actress Jenna Russell
Viewers immediately flooded Twitter with screenshots of the subtitle fail.
"Subtitles going wild again?! Fully went from 'fat little girl' to 'fat sk*t'," one person tweeted.
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Another said: "Did anyone else notice how 'fat little girl' became 'fat sk*t' in the subtitles on EastEnders?"
The Walford residents suffered a horrific bus crash
The dramatic scenes on EastEnders last night were overshadowed by the subtitle gaffe
The disastrous gaffe comes less than a week after the BBC were left red-faced when they showed the wrong subtitles during President Donald Trump's inauguration.
Millions of viewers watching Donald Trump as he was sworn in as the 45th President of the United States were confused after the BBC mistakenly showed subtitles from a children's TV programme.
Last night's episode of the soap also came under fire from viewers for the insensitive bus crash storyline only a month after the Berlin terror attacks that saw 12 people killed after a truck was deliberately driven into crowds at a Christmas market.