People returning from Belgium, the Bahamas and Andorra from midnight on Thursday will have to quarantine at home for a fortnight, the Welsh Government has said.
It is expected the rest of the UK will follow suit shortly.
Belgium has one of the highest coronavirus case rates in Europe, at 49.2 per 100,000 people, compared with 14.3 per 100,000 in the UK.
The changes were announced by Wales’ Health Minister Vaughan Gething.
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The three destinations will be removed from the list of countries which have been exempted from border health controls.
Luxembourg, Spain and Serbia were all removed last month.
Mr Gething said the decision was made after he “considered the evidence for the public health risk now posed by travellers who enter the UK from these places”.
In other changes, travellers arriving from Brunei and Malaysia will not need to quarantine.
A Welsh Government spokesman added: “The four nations of the UK made this decision together and we have amended our regulations.
“We understand similar changes will be made in the other nations.”
There are currently no direct commercial flights to Belgium, the Bahamas or Andorra from Wales, but air passengers can connect via Paris or Amsterdam or use an English airport.