During a booze-fuelled post-eviction party, Bianca gestured to the 30-year-old former footballer to go to the toilet next to the bedroom.
As he went in, the former glamour model sprayed some perfume before knocking on the door.
Bianca then covertly slipped into the loo behind him and kissing noises were heard from their microphones.
The pair were briefly interrupted by Stacy Francis who entered the toilet and left smiling.
Bianca Gascoigne shared a sneaky kiss with Jamie O'Hara in the Celebrity Big Brother toilet
Bianca led Jamie into the toilet after an evening of boozing
What are you talking about?
Later on Stacy asked Bianca: "Was it good?"
To which she responded: "What are you talking about?"
Jamie, who is the ex-husband of fellow CBB star Danielle Lloyd, has previously confessed he has feelings for the 30-year-old strip club owner.
Stacy Francis went into the toilet and caught the pair
Get Quotes on Home Insurance
Jamie and Bianca emerged from the toilet separately acting as if nothing had happened
Celebrity Big Brother 2017 highlights
Tue, January 17, 2017
Celebrity Big Brother 2017: The best bits from the latest series including all the arguments, kisses and Tony the Pony.
1 of 14
Celebrity Big Brother viewers were surprised to hear Kim Woodburn urging Chloe Ferry to become an escort
He said: “Bianca is really nice, beautiful and competitive, I like that in a woman.
"I like her. I’m holding back… she’s such a nice girl and down to earth."
"I really like that… I don’t know how she feels about me."
Celebrity Big Brother continues tomorrow night at 9pm on Channel 5.