The 35-year-old glamour model fell foul of The Hills' stars on Friday when she chose Coleen Nolan, 51, to win eternal immunity.
Spencer, 33, and Heidi, 30, who were then chosen to face every single eviction, were upset Nicola had chosen the Loose Women presenter over them.
Speidi and Nicola have become very close in recent days, which is why their ensuing row yesterday came as a surprise.
Things showed no sign of settling down on Day 19 despite Nicola's efforts at making peace.
The mother-of-two told them: “I’m absolutely devastated. You’re the last people in here I would want to hurt or offend.
Nicola McLean had another row with Spencer and Heidi Pratt on Celebrity Big Brother
Spencer rebuffed Nicola's attempts at trying to make peace with him and Heidi
“I would never have put you up for a killer nomination. I don’t want to row with you and I don’t want this beef with you because I really like you and you’ve been a massive part of this house."
However, Spencer was having none of it and hit back: “I was done with you the second you held up Coleen’s face. I don’t have a beef, just whatever you say from here on out to me, I don’t believe you one bit.
“That’s where I’m at. Heidi can do whatever she wants for you.”
After rowing further, Nicola headed to the smoking area, where she was joined by Jamie O'Hara and the source of the argument, Coleen.
Nicola chose Coleen Nolan to win eternal immunity over Speidi leading to tension
Nicola appeared to take her frustration out on Jamie O'Hara in the smoking area
Celebrity Big Brother 2017 highlights
Tue, January 17, 2017
Celebrity Big Brother 2017: The best bits from the latest series including all the arguments, kisses and Tony the Pony.
1 of 14
Celebrity Big Brother viewers were surprised to hear Kim Woodburn urging Chloe Ferry to become an escort
Nicola hinted she wanted to leave, saying: "I'm done."
Coleen attempted to explain the situation to Jamie, telling him that Nicola feels people have been “disloyal” by continuing to speak to Spencer and Heidi.
Despite his attempts to reassure her this was not the case, Nicola continued: “Just so you know, I won’t sit with people that make people feel how I’ve felt.”
Celebrity Big Brother continues tonight at 9pm on Channel 5.