Rail union members have rejected a deal aimed at resolving the dispute
Aslef said a proposed resolution to the dispute was rejected by 54.1 per cent in a turnout of 72 per cent.
General secretary Mick Whelan said: "We understand and support the decision arrived at democratically by our members and will now work to deliver a resolution in line with their expectations."
The proposal was rejected by over half of the Aslef members
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We understand and support the decision arrived at democratically by our members
General secretary Mick Whelan
Aslef said 953 ballot papers were sent out, with 693 returned. There were 317 votes in favour (45.9per cent) of the proposed deal, and 374 against (54.1per cent), with two invalid papers.
The union's leaders had agreed the deal during 11 days of talks held at the headquarters of the TUC, which ended earlier this month.
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Members of the Rail, Maritime and Transport union are also embroiled in a bitter row with Southern over staffing and are set to stage a 24-hour strike on February 22.
Southern staff look set to strike again on the 22 February
Nick Brown, Chief Operating Officer of Southern's owners, Govia Thameslink Railway, said: "Naturally we're saddened and hugely disappointed, as will be our passengers, with today's decision by drivers, particularly as the agreement carried the full support and recommendation of the Aslef leadership.
"We now need to understand the issues which led to this outcome and we'll be seeking to meet with the union as soon as possible to see how we can agree a way forward."