A repeated episode, shown on June 13 at 2.30pm, failed to clock one viewer, receiving a dreaded "zero" rating on an audience measuring system, BARB.
The episode's original airing which was broadcast on June 8 at 7.30pm gained 53,100 viewers.
In its heyday, the BBC hit would capture an audience of around eight million.
Despite the worrying figures, the BBC is standing firmly behind its flagship show, calling it "a national institution".
The current presenting trio of Blue Peter
The show has been on the BBC for almost 60 years
I challenge any naysayers to check out last week's enthralling episode on iPlayer if they have any doubts at all about its value and enduring appeal to the curious and clued-up CBBC audience
CBBC controller Cheryl Taylor told Express.co.uk: "It is very important to put the comments made about viewing figures for Blue Peter in context.
"The episode in question is our repeated 'signed' version which needs to air on CBBC in order to appear on BBC iPlayer – an important service for viewers who use BSL.
"An additional repeat of this episode in a different time slot had an average audience of 46,000 and a 10.3% share of children aged 6-12 which is above the slot average.
"It has also been viewed 39,000 times on BBC iPlayer so far," the statement added.
This comes after CBBC promised yesterday to spend £34.4 million on brand new children's content over the next three years.
Child stars then & now Sat, June 10, 2017
These child stars are all grown up. From Daniel Radcliffe, to the cast of Willy Wonka, see the children who aged gracefully into adulthood.
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Mara Wilson played the little girl in Matilda and Mrs Doubtfire
It is thought that the move is an attempt to move youngsters away from shows produced in America.
One children's show that has proven a big hit in recent months is the Bedtime Stories series.
The episodes have attracted the likes of Hollywood star Tom Hardy to read a sweet tale to adoring fans.
Express.co.uk has contacted a representative from the BBC for further comment.
Blue Peter continues tomorrow on CBBC at 5.30pm.