Neymar was sent off as Barcelona slumped to a 2-0 defeat to Malaga at the weekend.
However, the Catalan giants appealed in the hope he would be available for their title showdown with Real Madrid on April 23.
But he has lost that appeal and will now serve a two-game suspension, dealing a blow to Barcelona's hopes of winning La Liga in the process.
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One of the bans is because Neymar picked up two yellow cards.
And he will also miss a game against Real Sociedad after he sarcastically clapped the decision of the referee.
Barcelona will have to make do without the Brazil international when they visit the Santiago Bernabeu.
However, Neymar is set to feature in their Champions League showdown with Juventus tomorrow (7.45pm).