Barcelona are aiming to win the Champions League for a fourth time in the past seven years.
However, they go into the clash under a cloud.
The Catalan giants slumped to a shock 2-0 defeat to Malaga at the weekend.
Lionel Messi wants Barcelona to drop two players Barcelona transfer odds: Which Premier League stars could head to La Liga? Wed, March 29, 2017
Which Premier League players are most likely to join Barcelona in the summer, according to odds provided by 888sport?
Play slideshow Getty Images 1 of 11
Which Premier League players are most likely to join Barcelona this summer?
It means they trail La Liga leaders and fierce rivals Real Madrid by three points at the summit, albeit having played one game more.
With the title looking beyond them, it has placed extra significance on their showdown with Serie A leaders Juventus.
And Spanish outlet Don Balon claim Messi has asked manager Luis Enrique to not pick Mathieu and Gomes for the tie.
The Argentina international is not Gomes' biggest fan, with reports in Spain claiming he wants the midfielder sold in the summer.
Andre Gomes has endured a frustrating season at Barcelona
Gomes has struggled to adjust to life at the Nou Camp since arriving from Valencia last summer.
Get Quotes on Home Insurance
However, the report claims the Portugal international is not the only player Messi has an issue with.
It is said the Barcelona star also harbours doubts over Mathieu's ability to keep Juventus' Gonzalo Higuain quiet.
Higuain has scored 27 goals for Juventus this season and Messi knows all about him, given they are team-mates at international level.