Members of the Rail, Maritime and Transport (RMT) union will walk out on Friday April 28 after talks failed to break the deadlocked dispute.
Its members at the three operators staged a 24-hour strike last Saturday, the day of the Grand National.
The union has taken 31 days of strike action against Southern in the past year, although fresh talks will be held later this month to try to break the deadlock.
WALK-OUT: RMT Union have announced a 24-hour strike on April 28 over driver-only trains
Safety issues… leave us with no option but this further day of strike action
Mike Cash, RMT general secretary
Talks about staffing on new Merseyrail trains are also due to go ahead.
Strike action is part of a union campaign against new driver-controlled trains being introduced from 2020.
RMT general secretary Mick Cash said: "The public, who support RMT's campaign for a guarantee of a guard on their trains, will be appalled that Arriva Rail North have failed to offer any kind of progress whatsoever in the talks and have instead opted to try and bulldoze through their plans regardless.
"It is that flagrant disregard for the safety issues at the heart of the dispute which leaves us with no option but to put on this further day of strike action.
"RMT commends our Arriva Rail North members on their rock-solid display of strength, resolve and unity during the action so far in opposition to the introduction of driver-controlled operation and defence of the guards to guarantee a safer, securer and more accessible railway for all.
"The union continues to remain available for meaningful discussions with the company in order to seek a satisfactory resolution to the ongoing dispute."
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